2024, Vol. 12, Issue 3, Part C

author(s)
Md. Niaj Morshed, Md. Jakaria Islam, Md. Ripon Shikder, Md. Ibnul Piash, Md. Shahin Mia, Md. Saklaine Mostak, Md. Mehedi Hasan, Md. Ariful Islam, Toufiq Ejaj Khan and Imran Mahmud

abstract
In South Asian countries, Barleria lupulina Lindl is used ethno-medically to treat rheumatoid arthritis, diabetes, fever, pain, mental illness, and snake bites. This work aims to examine the pharmacological activities of ethanolic leaf extract of B. lupulina, including anthelminthic, antioxidant, and protein denaturation activity, with phytochemical screening. Secondary metabolites such as carbohydrates, tannins, flavonoids, saponins, steroids, glycosides, and quinones were identified by preliminary phytochemical tests. The ethanol extract of B. lupulina leaves showed considerable free radical scavenging activity (IC50 = 8.65 µg/ml) in the quantitative assay, compared to ascorbic acid (IC50 = 4.72 µg/ml) as a standard. The total phenolic content (TPC) of B. lupulina was measured at 153±0.11 µg GAE/g, whereas the total flavonoid content (TFC) was 32.26 ± 0.09 µg QE/g dry weight extract. Additionally, the anthelmintic properties of B. lupulina leaves against Paramphistomum cervi revealed encouraging outcomes at 200 µg/ml, compared to the reference standard (Albendazole). Moreover, Egg albumin denaturation was used to measure the protein denaturation activity. B. lupulina leaves extract and Diclofenac Na (standard) demonstrated percentage inhibition in the protein denaturation test of 53.78%, 55.25%, 60.6%, and 63.3%, 75%, 79%, respectively. Consequently, additional research is required to isolate and characterize the pure chemicals found in the extract, as they may provide a natural remedy for oxidative stress.